Small (short) RNAs.
Exploration of biogenesis via: assessing tiRNA concentrations associated with nuclear genetic material, accomplished by sifting nuclear and cytoplasmic fractions from THP-1 (a human monocytic cell line) and from mouse granulocytes for 15 to 30 nt RNAs; seeking evidence from THP-1 of nuclear locations for expressed small splice-site (3') RNAs (spliRNAs); clarifying from libraries that the observation for small spliRNAs held good for evolutionary distant metazoa; found no evidence of spliRNA in two budding yeasts nor in A. thaliona; identified miRNAs and moRNAs in THP-1 cells, and expression profiling showed some, though a minority, were from the nucleus. Emphasis added by HG.
This paper was published in Nature Structural Biology on 11th June, 2010. This item in development is the first test piece for Snippets of science. Source: "Nuclear-localized tiny RNAs are associated with transcription inititiation and splice sites in Metazoans.doi:10.1038/nsmb.1841. The above ought to have given the journal title as Nature Structural and Molecular Biology. The subject matter probably distracted me. The DOI is correct. The journal article is correct and the authors are Ryan T Taft et al. I would be grateful if readers could bear with me as I think through in practice exactly how this title is going to accomplish its aims expressed below*. There is only one item in this issue.
SELECTING FROM INTERNATIONAL ENGLISH SCIENCE JOURNALS IN SUPPORT OF BIOPHYSICS, FROM MACROMOLECULAR TO SUB ATOMIC AND FUNDAMENTAL PARTICLES. |
Snippets of science by Helen Gavaghan